Rhesus Macaque IFN-gamma Antibody

R&D Systems, part of Bio-Techne | Catalog # AF961

R&D Systems, part of Bio-Techne
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AF961
AF961-SP
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BAF961
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Key Product Details

Species Reactivity

Rhesus Macaque

Applications

ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IFN-gamma Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rhesus macaque IFN-gamma (R&D Systems, Catalog # 961-RM)
Gln24-Gln165
Accession # P63310

Specificity

Detects rhesus macaque IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 30% cross-reactivity with recombinant human IFN‑ gamma is observed and less than 0.1% cross-reactivity with recombinant mouse IFN‑ gamma, recombinant rat IFN‑ gamma, recombinant porcine IFN‑ gamma, recombinant canine IFN‑ gamma, recombinant cotton rat IFN‑ gamma, and recombinant feline IFN‑ gamma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rhesus Macaque IFN-gamma Antibody

IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Primate IFN-gamma Antibody.

IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Primate IFN-gamma Antibody.

Recombinant Rhesus Macaque IFN-gamma (Catalog # 961-RM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rhesus Macaque IFN-gamma (200 ng/mL) is neutralized (green line) by increasing concentrations of Rhesus Macaque IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961). The ND50 is typically 1.5-5.0 µg/mL.

Applications for Rhesus Macaque IFN-gamma Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rhesus Macaque IFN-gamma (Catalog # 961-RM)

Neutralization

Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 1.5-5.0 µg/mL in the presence of 200 ng/mL Recombinant Rhesus Macaque IFN‑ gamma.

Rhesus Macaque IFN-gamma Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Rhesus Macaque IFN-gamma Biotinylated Antibody (Catalog # BAF961)
  • Standard: Recombinant Rhesus Macaque IFN-gamma Protein (Catalog # 961-RM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rhesus IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid sequence identity with human IFN-gamma, 57-66% with bovine, canine, equine, feline, and porcine IFN-gamma, and 37-44% with cotton rat, mouse, and rat IFN‑ gamma. IFN‑ gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References

  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Villinger, F. et al. (1995) J. Immunol. 155:3946.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol

IFNG

UniProt

P63310

Additional IFN-gamma Products

Product Documents for Rhesus Macaque IFN-gamma Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rhesus Macaque IFN-gamma Antibody

For research use only

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