Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Retinoblastoma binding protein 1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 900 and 950 of human AT-rich interactive domain-containing protein 4A using the numbering given in entry NP_002883.3 (GeneID 5926).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Retinoblastoma binding protein 1 Antibody - BSA Free

Western Blot: Retinoblastoma binding protein 1 Antibody [NBP1-26616]

Western Blot: Retinoblastoma binding protein 1 Antibody [NBP1-26616]

Western Blot: Retinoblastoma binding protein 1 Antibody [NBP1-26616] - Detection of human ARID4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID4A antibody NBP1-26616 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Retinoblastoma binding protein 1 Antibody [NBP1-26616]

Immunoprecipitation: Retinoblastoma binding protein 1 Antibody [NBP1-26616]

Immunoprecipitation: Retinoblastoma binding protein 1 Antibody [NBP1-26616] - Detection of human ARID4A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID4A antibody NBP1-26616 used for IP at 3 ug per reaction. ARID4A was also immunoprecipitated by another rabbit anti-ARID4A antibody. For blotting immunoprecipitated ARID4A, NBP1-26616 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Retinoblastoma binding protein 1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Retinoblastoma binding protein 1

ARID4A (AT-rich interactive domain-containing protein 4A) is also known as RbBP1 (retinoblastoma binding protein 1) and is a retinoblastoma binding protein and member of the AT-rich interaction domain (ARID) family of proteins. In addition to the ARID DNA binding domain, ARID4A also contains a chromo domain and a TUDOR domain, indicating a function in chromatin organization and protein-protein interactions with methylated protein substrates. ARID4A is implicated to be involved in chromatin remodeling and epigenetic modifications and to play a role in the pathogenesis of cancer.

Alternate Names

AT rich interactive domain 4A (RBP1-like), RBBP-1, RBP-1, RBP1ARID domain-containing protein 4A, retinoblastoma binding protein 1, Retinoblastoma-binding protein 1RBBP1AT-rich interactive domain-containing protein 4A

Entrez Gene IDs

5926 (Human)

Gene Symbol

ARID4A

UniProt

NP_002883.3 (Human)

Additional Retinoblastoma binding protein 1 Products

Product Documents for Retinoblastoma binding protein 1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Retinoblastoma binding protein 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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