RBCK1 Antibody - BSA Free

Western Blot: RBCK1 Antibody - BSA Free [NBP1-88301]

Analysis in human cell lines SK-MEL-30 and HeLa using Anti-RBCK1 antibody. Corresponding RBCK1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Western Blot: RBCK1 Antibody - BSA Free [NBP1-88301] -

Expression of RNF31 in HCC cell lines and evaluation of RNF31 knockdown. (a) The mRNA level of RNF31 was evaluated in the HepG2, Hep3B, PLC, Huh7, HLE, and HLF cell lines by qPCR. mRNA level of RNF31 in Huh7 cells was used as reference. (b) The protein expression levels of RNF31, RBCK1, and SHARPIN were evaluated by Western blotting. (c) The effect of RNF31 knockdown was evaluated using Western blotting for HepG2, Hep3B, and PLC cells transfected with control (siCont) and RNF31-specific siRNAs (siRNA1-3). For quantification, normalized RNF31/b-actin level in parental cells was used as reference. d. The effect of RNF31 knockdown was evaluated using qPCRs. mRNA level of RNF31/GAPDH in control cells was used as reference. *P < 0.05; ns, non-specific; n.s., not significant; siCont, control siRNA. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-023-50594-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RBCK1 Antibody - BSA Free [NBP1-88301] -

Inhibition of RNF31 with HOIPIN-8 decreases proliferation, invasion, and NF-kappa B activation in HCC cell lines. (a) Cell proliferation was evaluated in HepG2 and Hep3B cells treated with 100 uM HOIPIN-8. (b) Invasion was evaluated in HepG2 and Hep3B cells in the presence of 10 or 30 uM HOIPIN-8. Representative images of invading cells are shown in the right panel. Cell nuclei are stained in purple. (c) Expression of NF-kappa B target genes in HepG2 and Hep3B cells was evaluated by qPCR. Cells were pretreated with 30 uM HOIPIN-8 for 1 h, followed by stimulation with 10 ng/mL TNF-alpha for 2 h. (d) Phosphorylation of NF-kappa B signaling factors upon TNF-alpha stimulation was evaluated in HepG2 cells with or without 30 uM HOIPIN-8. Cell lysates were immunoblotted by the indicated antibodies. (e) Effect of HOIPIN-8 on TNF-alpha + CHX-mediated apoptosis in HepG2 and Hep3B cells. Cells were pre-treated with HOIPIN-8 for 1 h and then stimulated with 40 ng/mL TNF-alpha and 20 ug/mL CHX for 0, 4, and 8 h, and cell lysates were immunoblotted by the indicated antibodies. *P < 0.05; n.s., not significant; Cas-3, caspase-3; cl-Cas-3, cleaved caspase-3; CHX, cycloheximide. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-023-50594-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.