RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41201
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Applications
Validated:
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Cited:
Western Blot, Chromatin Immunoprecipitation (ChIP), Chemotaxis, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 13D10
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The complete coding region (amino acids 1-425) of RBBP4/RbAp48 expressed in E. coli.
Reactivity Notes
Use in Rat reported in scientific literature (PMID:34205080).
Localization
Nuclear
Specificity
This antibody will recognize human RbAp48.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free (NBP1-41201) is a monoclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. Anti-RBBP4/RbAp48 Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free
![Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201]](https://resources.bio-techne.com/images/products/RBBP4-RbAp48-Antibody-13D10-Immunoprecipitation-NBP1-41201-img0017.jpg "Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201]")
Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201]
Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -
Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -
RbAp48 antibody [13D10] detects RbAp48 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RbAp48 protein stained by RbAp48 antibody [13D10] (NBP1-41201) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Applications for RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Reactivity reported in (PMID: 30551478)
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
Assay dependent
Immunoprecipitation
1:500
Western Blot
1:500-1:3000
Reviewed Applications
Read 1 review rated 5 using NBP1-41201 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RBBP4
Long Name
Retinoblastoma Binding Protein 4
Alternate Names
CAF-1 p48, CAF-1C, NURF55, RBAP48
Entrez Gene IDs
5928 (Human)
Gene Symbol
RBBP4
Additional RBBP4 Products
Product Documents for RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free
Product Specific Notices for RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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