RB1CC1 Antibody

Western Blot: RB1CC1 Antibody [NB100-77279]

Western Blot: RB1CC1 Antibody [NB100-77279] - Detection of Mouse FIP200/RB1CC1 by Western Blot. Samples: Whole cell lysate from TCMK-1 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FIP200/RB1CC1 antibody NB100-77279 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: RB1CC1 Antibody [NB100-77279]

Western Blot: RB1CC1 Antibody [NB100-77279] - Detection of Human FIP200/RB1CC1 on HeLa whole cell lysate using NB100-77279. FIP200/RB1CC1 was also immunoprecipitated by rabbit anti-FIP200/RB1CC1 antibody NB100-77317.

Western Blot: RB1CC1 Antibody [NB100-77279] -

EV-D68 and PV display similarities in virus production kinetics and interaction with canonical autophagy players. (A) Titers of both cell-associated and extracellular PV and EV-D68 from a time course of infection. H1HeLa cells were infected at an MOI of 20. Intracellular and extracellular virus samples were collected at each hour post-infection. Viral titers were analyzed by plaque assay. (B) Impact of enterovirus D68 and poliovirus on host autophagy markers. H1HeLa cells were either untreated/mock (M) or infected with PV or EV-D68 at an MOI of 20 for 6 h, and samples were collected every hour during infection. Samples were subjected to western blot analysis for traditional autophagy markers: LC3B, p62(SQSTM1), and its Cp (cleavage product). Viral marker: VP3 (virus structural capsid protein 3). Beta-actin served as a loading control. (C–E) Intracellular EV-D68 and PV titers produced in parental H1HeLa versus KO cell lines. H1HeLa WT or KOs were infected with PV or EV-D68 at an MOI of 20 for 6 h. Viral titers were analyzed by plaque assay. KO cell lines represented: RB1CC1 KO (C), PI3K3 KO (D), and ATG7 KO(E), respectively. Unpaired Student’s t test was used for the statistical analysis (***P < 0.001; **P < 0.01; *P ≤ 0.05; ns = not significant). The efficiency of KOs was assessed by western blot and the parental H1HeLa cell line (WT) was used as a control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37819109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.