Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human µ Opioid R/ OPRM1 Monoclonal Antibody (Catalog # MAB6866) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for µ Opioid R/OPRM1 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Rat μ Opioid R/OPRM1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Rat brain tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mu Opioid R/OPRM1

The mu-type opioid receptor (MOR), also known as OPRM1, is a 60‑70 kDa variably glycosylated G protein-coupled receptor that mediates the biological effects of many alkaloid and peptide opioids including morphine. MOR is primarily expressed on neurons in the brain, spinal cord, and gastrointestinal tract as well as on immune cells. MOR activation induces analgesia, euphoria, sedation, respiratory depression, and reduced intestinal motility. Following agonist binding, MOR is phosphorylated and internalized which contributes to opioid tolerance and desensitization. OPRM1 can form heterdimers with several other 7TM GPCRs including the delta-type Opioid Receptor (DOR), Nociceptin/Orphanin Receptor (ORL1), Neurokinin 1 Receptor (NK1), Somatostatin Receptor 2 (SSTR2), Cannabinoid Receptor 1, CCR5, and the alpha2A-Adrenergic Receptor (ADRA2A). Rat MOR shares 94% and 98% aa sequence identity with human and mouse MOR, respectively.

Long Name

mu Opioid Receptor

Alternate Names

LMOR, MOP, mOpioidR, MOR, MOR1, OPRM1

Entrez Gene IDs

4988 (Human); 18390 (Mouse); 25601 (Rat)

Gene Symbol

OPRM1

UniProt

P33535

Additional mu Opioid R/OPRM1 Products

Product Documents for Rat μ Opioid R/OPRM1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat μ Opioid R/OPRM1 Antibody

For research use only

Citations
Reviews

Rat μ Opioid R/OPRM1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6866

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all mu Opioid R/OPRM1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat μ Opioid R/OPRM1 Antibody

Detection of Rat μ Opioid R/OPRM1 by Western Blot.