Rat IL-6 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF506

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BAF506
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Human, Rat

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Capture, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all IL-6 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat IL-6
Phe25-Thr211
Accession # P20607

Specificity

Detects rat IL-6 in ELISAs and Western blots. In sandwich immunoassays, less than 0.8% cross‑reactivity with rmIL‑6 and less than 0.3% cross‑reactivity with rhIL‑6, rpIL‑6 and rrCNTF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat IL-6 Biotinylated Antibody

Rat IL-6 ELISA Standard Curve

Rat IL-6 ELISA Standard Curve

Recombinant Rat IL-6 (Catalog # 506-RL) was serially diluted and captured by Mouse Anti-Rat IL-6 Monoclonal Antibody (Catalog # MAB506) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Rat IL-6 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rat IL-6 (Catalog # 506-RL)

Rat IL-6 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Rat IL-6 Antibody (Catalog # MAB506)
  • Capture Reagent: Rat IL-6 Antibody (Catalog # MAB506R)
  • Standard: Recombinant Rat IL-6 Protein (Catalog # 506-RL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using BAF506 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature rat IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Rat IL-6 shares 39% and 85% aa sequence identity with human and mouse IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

UniProt

P20607

Additional IL-6 Products

Product Documents for Rat IL-6 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rat IL-6 Biotinylated Antibody

For research use only

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