Key Product Details

Species Reactivity

Rat

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all beta 2-Microglobulin Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat beta2-Microglobulin
Ile21-Met119
Accession # P07151

Specificity

Detects rat beta

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat beta 2-Microglobulin Antibody

Detection of beta2-Microglobulin antibody in Rat Splenocytes antibody by Flow Cytometry.

Detection of beta2-Microglobulin in Rat Splenocytes by Flow Cytometry.

Rat splenocytes were stained with Rat beta2-Microglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3864, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for Rat beta 2-Microglobulin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Rat splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Rat beta2-Microglobulin

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta 2-Microglobulin

beta2-Microglobulin ( beta2M) is a ubiquitous, 12 kDa, secreted, non-glycosylated protein required for cell surface expression and non-covalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat beta2M is a 99 amino acid (aa) peptide containing one C1-type lg-like domain (aa 22-116). In humans, beta2M is known to dissociate from the MHC complex and circulate as full-length and N-terminal-truncated peptides of 93, 91, and 90 amino acids. Mature rat  beta2M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively.

Alternate Names

B2M, beta 2Microglobulin

Entrez Gene IDs

567 (Human); 12010 (Mouse); 24223 (Rat); 101867173 (Cynomolgus Monkey)

Gene Symbol

B2M

UniProt

P07151

Additional beta 2-Microglobulin Products

Product Documents for Rat beta 2-Microglobulin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Rat beta 2-Microglobulin Antibody

For research use only

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