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Rab7a Antibody (24GB895)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51463

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NBP3-51463-100ul
NBP3-51463-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB895

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human Rab7a (Uniprot: P51149)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rab7a Antibody (24GB895)

Rab7a Antibody (24GB895)

Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (24GB895)[NBP3-51463] -

Immunocytochemical staining of H9c2 cells with Rab7a antibody (1:1,000) . Nuclei were stained blue with DAPI; Rab7a was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
Rab7a Antibody (24GB895)

Flow Cytometry: Rab7a Antibody (24GB895)[NBP3-51463] -

Validation of Rab7a knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Rab7a antibody (1:2,000) and analyzed using BD flow cytometer.
Rab7a Antibody (24GB895)

Flow Cytometry: Rab7a Antibody (24GB895)[NBP3-51463] -

Flow cytometric analysis of Rab7aexpression in H9c2 cells using Rab7a antibody (1:2,000). Green, isotype control; red, RAB7A.

Applications for Rab7a Antibody (24GB895)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rab7a

RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

Long Name

RAs Genes from Brain Protein 7a

Alternate Names

PRO2706, RAB7

Gene Symbol

RAB7A

Additional Rab7a Products

Product Documents for Rab7a Antibody (24GB895)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rab7a Antibody (24GB895)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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