Pyruvate Kinase Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44231
Key Product Details
Species Reactivity
Rabbit
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Pyruvate Kinase (Rabbit Muscle) (Uniprot: P11974)
Reactivity Notes
Cross-reactivity against pyruvate kinase from other mammalian tissues is expected but has not been specifically determined.
Specificity
This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross-reactivity against pyruvate kinase from other mammalian tissues is expected but has not been specifically determined.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pyruvate Kinase (Rabbit Muscle)
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Pyruvate Kinase Antibody - BSA Free
![Western Blot: Pyruvate Kinase Antibody [NBP2-44231]](https://resources.bio-techne.com/images/products/Pyruvate-Kinase-Antibody-Western-Blot-NBP2-44231-img0001.jpg "Western Blot: Pyruvate Kinase Antibody [NBP2-44231]")
Western Blot: Pyruvate Kinase Antibody [NBP2-44231]
Western Blot: Pyruvate Kinase Antibody [NBP2-44231] - Western Blot of Goat Pyruvate Kinase antibody. Lane 1: CHO K1 Lysate [10ug].Lane 2: CHO S Lysate [10ug]. Lane 3: Opal Pre-stained Molecular Weight Marker. Primary Antibody: Anti-Pyruvate Kinase at 1:50 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG CY5 conjugate at 1:10,000 for 30 mins at RT.Expect: ~57kDa.Applications for Pyruvate Kinase Antibody - BSA Free
Application
Recommended Usage
ELISA
1:4000-1:20000
Immunohistochemistry
1:500-1:2000
Western Blot
1:500-1:2000
Application Notes
This affinity purified antibody has been tested in western blot. This antibody is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Pyruvate Kinase
Alternate Names
PKM, PKM2
UniProt
Additional Pyruvate Kinase Products
Product Documents for Pyruvate Kinase Antibody - BSA Free
Product Specific Notices for Pyruvate Kinase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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