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PRMT7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19939

Novus Biologicals, part of Bio-Techne
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NBP2-19939

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT7. The exact sequence is proprietary.

Reactivity Notes

Bovine (83%)

Localization

Cytoplasm; cytosol; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PRMT7 Antibody (NBP2-19939) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. Anti-PRMT7 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PRMT7 Antibody

Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939]

Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939]

Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
PRMT7 Antibody

Immunocytochemistry/ Immunofluorescence: PRMT7 Antibody [NBP2-19939] -

PRMT7 antibody detects PRMT7 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRMT7 protein stained by PRMT7 antibody (NBP2-19939) diluted at 1:500.
Blue: Hoechst 33342 staining.
PRMT7 Antibody

Immunoprecipitation: PRMT7 Antibody [NBP2-19939] -

PRMT7 antibody immunoprecipitates PRMT7 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 ug of PRMT7 antibody (NBP2-19939) 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody (NBP2-19939) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Applications for PRMT7 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRMT7

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM]

Long Name

Protein arginine N-methyltransferase 7

Alternate Names

EC 2.1.1.321, KIAA1933

Gene Symbol

PRMT7

UniProt

Additional PRMT7 Products

Product Documents for PRMT7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRMT7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.