POLR3B Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84628
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VEADRKGAVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQ
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for POLR3B Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: POLR3B Antibody [NBP1-84628]](https://resources.bio-techne.com/images/products/POLR3B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84628-img0007.jpg "Immunocytochemistry/ Immunofluorescence: POLR3B Antibody [NBP1-84628]")
Immunocytochemistry/ Immunofluorescence: POLR3B Antibody [NBP1-84628]
Immunocytochemistry/Immunofluorescence: POLR3B Antibody [NBP1-84628] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.![Immunohistochemistry-Paraffin: POLR3B Antibody [NBP1-84628]](https://resources.bio-techne.com/images/products/POLR3B-Antibody-Immunohistochemistry-Paraffin-NBP1-84628-img0006.jpg "Immunohistochemistry-Paraffin: POLR3B Antibody [NBP1-84628]")
Immunohistochemistry-Paraffin: POLR3B Antibody [NBP1-84628]
Immunohistochemistry-Paraffin: POLR3B Antibody [NBP1-84628] - Staining of human rectum shows strong nuclear/ or nucleolar positivity in glandular cells.![POLR3B Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: POLR3B Antibody - BSA Free [NBP1-84628]](https://resources.bio-techne.com/images/products/nbp1-84628_rabbit-polyclonal-polr3b-antibody-2562025913939.jpg "Chromatin Immunoprecipitation-exo-Seq: POLR3B Antibody - BSA Free [NBP1-84628]")
Chromatin Immunoprecipitation-exo-Seq: POLR3B Antibody - BSA Free [NBP1-84628]
ChIP-Exo-Seq composite graph for Anti-POLR3B (NBP1-84628) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for POLR3B Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: POLR3B
Alternate Names
C128, DKFZp686B10117, DNA-directed RNA polymerase III 127.6 kDa polypeptide, DNA-directed RNA polymerase III subunit B, DNA-directed RNA polymerase III subunit RPC2, EC 2.7.7.6, FLJ10388, polymerase (RNA) III (DNA directed) polypeptide B, RNA polymerase III subunit C2, RPC2
Gene Symbol
POLR3B
Additional POLR3B Products
Product Documents for POLR3B Antibody - BSA Free
Product Specific Notices for POLR3B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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