Pollen allergen Phl p 1 Antibody (Clone 25) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81275
Key Product Details
Species Reactivity
Plant
Applications
Dot Blot, ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Human IgE Kappa Clone # Clone 25
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant Phl p 1, a grass pollen allergen.
Reactivity Notes
Expected to react with Timothy-Grass (Phleum pratense).
Specificity
This antibody binds to the Timothy grass allergen Phl p 1 and shows cotss reactivity with natural group 1 allergens from a variety of grass species such as oat, sweet vernal grass, Bermuda grass, rye grass, common reed, Kentucky blue grass, rye, wheat and maize (weak).
Clonality
Monoclonal
Host
Human
Isotype
IgE Kappa
Description
Novus Biologicals Human Pollen allergen Phl p 1 Antibody (Clone 25) - Azide and BSA Free (NBP2-81275) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Pollen allergen Phl p 1 Antibody (Clone 25) - Azide and BSA Free
![ELISA: Pollen allergen Phl p 1 Antibody (Clone 25) - Azide and BSA Free [NBP2-81275]](https://resources.bio-techne.com/images/products/Pollen-allergen-Phl-p-1-Antibody-Clone-25-ELISA-NBP2-81275-img0001.jpg "ELISA: Pollen allergen Phl p 1 Antibody (Clone 25) - Azide and BSA Free [NBP2-81275]")
ELISA: Pollen allergen Phl p 1 Antibody (Clone 25) - Azide and BSA Free [NBP2-81275]
ELISA: Pollen allergen Phl p 1 Antibody (Clone 25) [NBP2-81275] - Binding curve of Pollen allergen Phl p 1 Antibody (Clone 25) [NBP2-81275] to Phl p 1 ELISA Plate coated with Phl p 1 at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-81276. For detection, a 1:4000 dilution of HRP-labelled goat anti-human kappa antibody was used.Applications for Pollen allergen Phl p 1 Antibody (Clone 25) - Azide and BSA Free
Application
Recommended Usage
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to Phl p 1 and competes with up to 43.4% (mean 19.8%) of IgE from allergic patient serum.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Pollen allergen Phl p 1
Alternate Names
Allergen Phl p I, grass pollen allergen
UniProt
Additional Pollen allergen Phl p 1 Products
Product Documents for Pollen allergen Phl p 1 Antibody (Clone 25) - Azide and BSA Free
Product Specific Notices for Pollen allergen Phl p 1 Antibody (Clone 25) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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