POLE4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49672

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NBP2-49672
NBP2-49672-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Chromatin Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLE4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GQEAIFILARAAELFVETIAKDAYCCAQQGKRKTLQRRDLDNAIEAVDEFAFLEGTLD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLE4 Antibody - BSA Free

Immunohistochemistry: POLE4 Antibody [NBP2-49672]

Immunohistochemistry: POLE4 Antibody [NBP2-49672]

Immunohistochemistry: POLE4 Antibody [NBP2-49672] - Staining of human kidney shows strong nuclear positivity in cells in tubules.
POLE4 Antibody - BSA Free Chromatin Immunoprecipitation ChIP: POLE4 Antibody - BSA Free

Chromatin Immunoprecipitation ChIP: POLE4 Antibody - BSA Free

ChIP-Exo-Seq composite graph for Anti-POLE4 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.

Applications for POLE4 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1-10µg per reaction

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLE4

POLE4, also known as DNA polymerase epsilon subunit 4, is 117 amino acids long and approximately 12kDa. POLE4 localizes to the nucleus. POLE, POLE2, POLE3 and POLE4 are elements of the epsilon DNA polymerase complex (Pol epsilon), and as such, POLE4 plays a role in the process of nuclear DNA replication. POLE4 interacts with POLE, POLE2, and POLE3, as well as UBE3A and HSPA13. POLE4 is implicated in a variety of pathways including the DNA replication pathway, Nucleotide excision repair, Purine metabolism, and cell cycle control of chromosomal replication.

Alternate Names

DNA polymerase epsilon p12 subunit, DNA polymerase epsilon subunit 4, DNA polymerase epsilon subunit p12, DNA polymerase II subunit 4, EC 2.7.7.7, p12, polymerase (DNA-directed), epsilon 4 (p12 subunit), YHHQ1

Gene Symbol

POLE4

Additional POLE4 Products

Product Documents for POLE4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for POLE4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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