Podocan Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93238
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 67-210 of human PODN (NP_714914.2). LGPVLAVRAPGFGRSGGHSLSPEENEFAEEEPVLVLSPEEPGPGPAAVSCPRDCACSQEGVVDCGGIDLREFPGDLPEHTNHLSLQNNQLEKIYPEELSRLHRLETLNLQNNRLTSRGLPEKAFEHLTNLNYLYLANNKLTLAP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Podocan Antibody - BSA Free
![Western Blot: Podocan AntibodyBSA Free [NBP2-93238]](https://resources.bio-techne.com/images/products/Podocan-Antibody-Western-Blot-NBP2-93238-img0001.jpg "Western Blot: Podocan AntibodyBSA Free [NBP2-93238]")
Western Blot: Podocan AntibodyBSA Free [NBP2-93238]
Western Blot: Podocan Antibody [NBP2-93238] - Analysis of extracts of various cell lines, using Podocan at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.Applications for Podocan Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Podocan
Podocan is a secreted glycoprotein belonging to the small leucine-rich repeat (SLR) protein family. It lacks a serine/glycine dipeptide consensus sequence required for glycosaminoglycan attachment and is not a proteoglycan. Podocan transcripts were originally isolated from kidney podocytes. Podocan is also expressed in multiple other tissues including pineal gland, bladder, and vascular tissues. Podocan binds type I collagen and may affect cell migration. The amino acid sequences of mature human and mouse podocan share 92% identity.
Alternate Names
PCAN, PODN, SLRR5A
Gene Symbol
PODN
Additional Podocan Products
Product Documents for Podocan Antibody - BSA Free
Product Specific Notices for Podocan Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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