PML Protein Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87783
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ISGFLAALPLIRERVPGASSFKLKNLAQTYLARNMSERSAMAAVLAMRDLCRLLEVSPGPQLAQHVYPFSSLQCFASLQPLVQAAVLPRAEARLLALHNVSFMELLSAHRRDRQGGLKKYSRYLSLQTTTLP
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (80%). Reactivity reported in scientific literature (PMID: 24194542)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PML Protein Antibody - BSA Free (NBP1-87783) is a polyclonal antibody validated for use in IHC and WB. Anti-PML Protein Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PML Protein Antibody - BSA Free
![Immunohistochemistry-Paraffin: PML Protein Antibody [NBP1-87783]](https://resources.bio-techne.com/images/products/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NBP1-87783-img0006.jpg "Immunohistochemistry-Paraffin: PML Protein Antibody [NBP1-87783]")
Immunohistochemistry-Paraffin: PML Protein Antibody [NBP1-87783]
Immunohistochemistry-Paraffin: PML Protein Antibody [NBP1-87783] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.![PML Protein Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: PML Protein Antibody - BSA Free [NBP1-87783]](https://resources.bio-techne.com/images/products/nbp1-87783_rabbit-polyclonal-pml-protein-antibody-2562025993220.jpg "Chromatin Immunoprecipitation-exo-Seq: PML Protein Antibody - BSA Free [NBP1-87783]")
Chromatin Immunoprecipitation-exo-Seq: PML Protein Antibody - BSA Free [NBP1-87783]
ChIP-Exo-Seq composite graph for Anti-PML (NBP1-87783) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for PML Protein Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.. Use in Western blot reported in scientific literature (PMID 28095508).
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PML
Long Name
Promyelocytic Leukemia
Alternate Names
MYL, RNF71, TRIM19
Gene Symbol
PML
Additional PML Products
Product Documents for PML Protein Antibody - BSA Free
Product Specific Notices for PML Protein Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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