How many test can be performed in 0.1 ml of PLA2R1 Antibody (NBP1-84449). Also kindly suggest the secondary Antibody for the same.

Name: PLA2R1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-84449

The number of tests using NBP1-84449 will depend upon the sample type (differential tissues expression) and the total volume of antibody used in each slide. This product is supplied in 100ul size and the concentration of the antibody is 0.4ug/ul and the working dilution we recommend for IHC is 1:500 to 1:1000, based on this information you should be able to estimate how many experiments can be done with one vial. As far as secondary antibody is concerned, any anti rabbit IgG secondary that is conjugated should technical work, the type of conjugation does not matter as much, HRP is most often used in labs while the biotin-conjugated antibody is used to amplify the signal which results in greater sensitivity than that achieved with an enzyme or fluorescence conjugated secondary antibody alone. Fluorescent labeled antibodies are commonly used for double or multiple staining methods. Here is the list of our secondary antibodies. I can recommend NB730-H as it has received very good reviews from our customers and it has been cited in 23 publications as well. I hope this answers your question. Please do not hesitate to contact us if you have any further questions.

PLA2R1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84449

Submit a review

Citations (10)

Product Datasheet / COA / SDS

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PLA2R1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EEKTWHEALRSCQADNSALIDITSLAEVEFLVTLLGDENASETWIGLSSNKIPVSFEWSNDSSVIFTNWHTLEPHIFPNRSQLCVSAEQSEGHWKVKNCEERLFYICKKAGHVLSDAESGCQEGWERHGGFCYKID

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit PLA2R1 Antibody - BSA Free (NBP1-84449) is a polyclonal antibody validated for use in IHC and WB. Anti-PLA2R1 Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PLA2R1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining in human kidney and pancreas tissues . Corresponding PLA2R1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining o human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.

View 2 more images

Applications for PLA2R1 Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLA2R1

Secretory phospholipases A2 (PLA2s) have been purified from a variety of mammalian tissues as well as from insect and snake venoms. The prototype group I PLA2, the pancreatic PLA2 (PLA2G1B; MIM 172410), is involved in digestion, smooth muscle contraction, and cell proliferation. The prototype group II PLA2, the inflammatory-type PLA2 (PLA2G2A; MIM 172411), is involved in inflammatory conditions and is upregulated by proinflammatory cytokines like tumor necrosis factor (TNF; MIM 191160) and interleukin-1 (e.g., IL1B; MIM 147720). Differential toxicity of snake venom Pla2 appears to be linked to a variety of high-affinity receptors in different organs (Ancian et al., 1995 (PubMed 7721806)).(supplied by OMIM)

Long Name

Phospholipase A2 Receptor 1, 180kDa

Alternate Names

CLEC13C, PLA2G1R

Entrez Gene IDs

22925 (Human)

Gene Symbol

PLA2R1

UniProt

Q13018 (Human)

Additional PLA2R1 Products

Product Documents for PLA2R1 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for PLA2R1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

PLA2R1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84449

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all PLA2R1 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Description

Scientific Data Images for PLA2R1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449]

Western Blot: PLA2R1 Antibody - BSA Free [NBP1-84449]

Applications for PLA2R1 Antibody - BSA Free

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: PLA2R1

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional PLA2R1 Products

Product Documents for PLA2R1 Antibody - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for PLA2R1 Antibody - BSA Free