PLA2R1 Antibody (CL0474)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52061
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # CL0474
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q13018, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PLA2R1 Antibody (CL0474)
Western Blot: PLA2R1 Antibody (CL0474) [NBP3-52061]
Analysis in human kidney tissue.Immunohistochemistry: PLA2R1 Antibody (CL0474) [NBP3-52061]
Staining of human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (CL0474) [NBP3-52061]
Staining of Hela and MCF7 exhibiting a relatively high and low expression of PLA2R1 (based on RNA-seq values), respectively. The PLA2R1 (green signal) is present in Hela cells but is absent in MCF7 cells.Applications for PLA2R1 Antibody (CL0474)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:20000 - 1:50000
Immunohistochemistry-Paraffin
1:20000 - 1:50000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PLA2R1
Long Name
Phospholipase A2 Receptor 1, 180kDa
Alternate Names
CLEC13C, PLA2G1R
Gene Symbol
PLA2R1
Additional PLA2R1 Products
Product Documents for PLA2R1 Antibody (CL0474)
Product Specific Notices for PLA2R1 Antibody (CL0474)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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