PKC zeta [p Thr560] Antibody (24GB890)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50571

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NBP3-50571-100ul
NBP3-50571-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB890

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKC zeta Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-PKC zeta (T560) (Uniprot: Q05513)

Modification

p Thr560

Specificity

This antibody detects endogenous levels of PRKCZ only when phosphorylated at T560

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC zeta [p Thr560] Antibody (24GB890)

PKC zeta [p Thr560] Antibody (24GB890)

Immunocytochemistry/ Immunofluorescence: PKC zeta [p Thr560] Antibody (24GB890)[NBP3-50571] -

Immunocytochemical staining of HepG2 cells with PKC zeta antibody (1:1,000) . Nuclei were stained blue with DAPI; PKC zeta was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
PKC zeta [p Thr560] Antibody (24GB890)

Flow Cytometry: PKC zeta [p Thr560] Antibody (24GB890)[NBP3-50571] -

Flow cytometric analysis of PKC zetaexpression in HepG2 cells using PKC zeta antibody (1:2,000). Green, isotype control; red, Phospho-PKC zeta (T560).
PKC zeta [p Thr560] Antibody (24GB890)

Western Blot: PKC zeta [p Thr560] Antibody (24GB890)[NBP3-50571] -

Western blotting analysis using PKC zeta (T560) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for PKC zeta [p Thr560] Antibody (24GB890)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKC zeta

Protein kinase C (PKC), a class of serine/threonine kinases activated by Ca2+ and/or phospholipids, is involved in a variety of cellular processes such as proliferation, differentiation and secretion. PKC zeta exhibits a constitutive kinase activity which is independent of Ca2+, phosphatidylserine and diacylglycerol. Arachidonic acid alone or a combination of gamma-linolenic acid and phosphatidylserine slightly enhance PKC zeta activity. PKC zeta, in contrast to PKC alpha, is insensitive to PKC inhibitors known to interfere either with the regulatory or the catalytic domain and cannot be activated by phorbol ester treatment of NIH 3T3 cells or insect cells, overexpressing the respective PKC isoenzyme (1). Results have identified PDK-1 as the kinase that phosphorylates and activates PKC zeta in the PI 3-kinase signaling pathway. This phosphorylation and activation of PKC zeta by PDK-1 is enhanced in the presence of Ptdins-3,4-5-P3. PKCs are enzymes that are regulated at the plasma membrane, and a membrane-targeted PKC zeta is constitutively active in the absence of agonist stimulation. The association between PKC zeta and PDK-1 reveals extensive cross-talk between enzymes in the PI 3-kinase signaling pathway. (2)

Long Name

Protein Kinase C zeta

Alternate Names

PKC2, PRKCZ

Gene Symbol

PRKCZ

Additional PKC zeta Products

Product Documents for PKC zeta [p Thr560] Antibody (24GB890)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PKC zeta [p Thr560] Antibody (24GB890)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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