PITX1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1546
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Paired-Like Homeodomain Transcription Factor 1 using the numbering given in entry NP_002644.3 (GeneID 5307).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PITX1 Antibody - BSA Free
![Western Blot: PITX1 Antibody [NB100-1546]](https://resources.bio-techne.com/images/products/PITX1-Antibody-Western-Blot-NB100-1546-img0003.jpg "Western Blot: PITX1 Antibody [NB100-1546]")
Western Blot: PITX1 Antibody [NB100-1546]
Western Blot: PITX1 Antibody [NB100-1546] - Detection of Human PITX by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PITX antibody NB100-1546 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
PITX1 Antibody [NB100-1546] - Detection of human PITX by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PITX antibody NB100-1546 (lot NB100-1546-2) used for IP at 6 ug per reaction. PITX was also immunoprecipitated by a previous lot of this antibody NB100-1546-1. For blotting immunoprecipitated PITX, A300-577A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Applications for PITX1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1-4 ug/mg of lysate
Western Blot
1:2500-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PITX1
Alternate Names
BFTpituitary homeobox 1, CCF, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, paired-like homeodomain 1, pituitary otx-related factor, POTXpituitary homeo box 1, PTX1Paired-like homeodomain transcription factor 1hindlimb expressed homeobox protein backfoot
Entrez Gene IDs
5307 (Human)
Gene Symbol
PITX1
UniProt
Additional PITX1 Products
Product Documents for PITX1 Antibody - BSA Free
Product Specific Notices for PITX1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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