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Phosphorylase B Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-47160

Novus Biologicals, part of Bio-Techne
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NBP3-47160

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

phosphorylase kinase, beta. (Uniprot# Q93100)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Phosphorylase B Antibody - BSA Free (NBP3-47160) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Phosphorylase B Antibody - BSA Free

Phosphorylase B Antibody - BSA Free

Immunohistochemistry: Phosphorylase B Antibody - BSA Free [NBP3-47160] -

Immunohistochemistry: Phosphorylase B Antibody - BSA Free [NBP3-47160] - Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using (Phosphorylase B Antibody) at dilution of 1:50
Phosphorylase B Antibody - BSA Free

Immunoprecipitation: Phosphorylase B Antibody - BSA Free [NBP3-47160] -

Immunoprecipitation: Phosphorylase B Antibody - BSA Free [NBP3-47160] - IP Result of anti-Phosphorylase B (IP:, 4ug; Detection: 1:500) with mouse heart tissue lysate 3200ug.
Phosphorylase B Antibody - BSA Free

Western Blot: Phosphorylase B Antibody - BSA Free [NBP3-47160] -

Western Blot: Phosphorylase B Antibody - BSA Free [NBP3-47160] - Mouse heart tissue were subjected to SDS PAGE followed by western blot with (Phosphorylase B antibody) at dilution of 1:1000

Applications for Phosphorylase B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phosphorylase B

Phosphorylase B, also known by its gene name, PHKB, is an enzyme localized to the plasma membrane that catalyzes the phosphorylation of the amino acid residue Serine. Phosphorylase B has a 1,093 amino acid long isoform that is 125 kDa (the canonical sequence) and three other isoforms derived from alternative splicing. Current research on PHKB is being performed relating to several diseases and disorders including hepatitis and metabolic disorders, such as hypoglycemia and glycogen storage disease. Phosphorylase B has also been shown to have interactions with B Raf, hnRNP C1 + C2, PASK, and UBE3A in pathways such as Glucose and Glycogen Metabolism, PKA Signaling, cAMP Pathway, Insulin Signaling Pathway and Calcium Signaling Pathway.

Alternate Names

DKFZp781E15103, FLJ41698, phosphorylase b kinase regulatory subunit beta, phosphorylase kinase beta-subunit, Phosphorylase kinase subunit beta, phosphorylase kinase, beta

Gene Symbol

PHKB

Additional Phosphorylase B Products

Product Documents for Phosphorylase B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Phosphorylase B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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