Phospho-Tyrosine Antibody (21) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42911
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 21
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to Phospho-Tyrosine
Reactivity Notes
Species independent
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
Novus Biologicals Mouse Phospho-Tyrosine Antibody (21) - Azide and BSA Free (NBP2-42911) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Phospho-Tyrosine Antibody (21) - Azide and BSA Free
![Western Blot: Phospho-Tyrosine Antibody (21) [NBP2-42911]](https://resources.bio-techne.com/images/products/Phospho-Tyrosine-Antibody-21-Western-Blot-NBP2-42911-img0001.jpg "Western Blot: Phospho-Tyrosine Antibody (21) [NBP2-42911]")
Western Blot: Phospho-Tyrosine Antibody (21) [NBP2-42911]
Western Blot: Phospho-Tyrosine Antibody (21) [NBP2-42911] - Analysis of Un-treated (-) and treated (+), 1mM Pervanadate treatment for 30min. Raji whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Phosphotyrosine antibody.
Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (21) [NBP2-42911] -
Phosphotyrosine antibody [GT0021] detects Phosphotyrosine protein at cytoplasm by immunofluorescent analysis.Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Phosphotyrosine protein stained by Phosphotyrosine antibody [GT0021] (NBP2-42911) diluted at 1:50.
Red: paxillin, a cytoskeleton marker, stained by Paxillin antibody diluted at 1:100.
Applications for Phospho-Tyrosine Antibody (21) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:1000
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Phospho-Tyrosine
Alternate Names
PhosphoTyrosine
Additional Phospho-Tyrosine Products
Product Documents for Phospho-Tyrosine Antibody (21) - Azide and BSA Free
Product Specific Notices for Phospho-Tyrosine Antibody (21) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...