PHGDH Antibody (CL0555) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43966

Novus Biologicals, part of Bio-Techne
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NBP3-43966
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BSA Free
NBP2-52939
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0555

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PHGDH Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O43175, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse PHGDH Antibody (CL0555) - Azide and BSA Free (NBP2-52939) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PHGDH Antibody (CL0555) - Azide and BSA Free

PHGDH Antibody (CL0555) - Azide and BSA Free Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-43966]

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-43966]

Analysis in human pancreas and skeletal muscle tissues using NBP3-43966 antibody. Corresponding PHGDH RNA-seq data are presented for the same tissues.
PHGDH Antibody (CL0555) - Azide and BSA Free Western Blot: PHGDH Antibody [NBP3-43966]

Western Blot: PHGDH Antibody [NBP3-43966]

Lane 1: Marker [kDa]
Lane 2: Human cell line RT-4
PHGDH Antibody (CL0555) - Azide and BSA Free Western Blot: PHGDH Antibody [NBP3-43966]

Western Blot: PHGDH Antibody [NBP3-43966]

Lane 1: Marker [kDa]
Lane 2: Human cell line HeLa cytoplasmic fraction
Lane 3: Human cell line HeLa membrane fraction
Lane 4: Human cell line HeLa nuclear fraction
Lane 5: Human cell line HeLa chromatin fraction
Lane 6: Human cell line HeLa cytoskeletal fraction
View 5 more images

Applications for PHGDH Antibody (CL0555) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHGDH

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Long Name

3-Phosphoglycerate Dehydrogenase

Alternate Names

3PGDH, PDGH3

Gene Symbol

PHGDH

Additional PHGDH Products

Product Documents for PHGDH Antibody (CL0555) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHGDH Antibody (CL0555) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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