PHF22 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58977
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SSSVTSGLTGWAAFAAKTSSAGPSTAKLSSTTQNNTGKPATSSANQKPVGLTGLATSSKGGIGSKIGSNNSTT
Reactivity Notes
Mouse 88%, Rat 89%
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PHF22 Antibody - BSA Free (NBP2-58977) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PHF22 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: PHF22 Antibody [NBP2-58977]](https://resources.bio-techne.com/images/products/PHF22-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58977-img0001.jpg "Immunocytochemistry/ Immunofluorescence: PHF22 Antibody [NBP2-58977]")
Immunocytochemistry/ Immunofluorescence: PHF22 Antibody [NBP2-58977]
Immunocytochemistry/Immunofluorescence: PHF22 Antibody [NBP2-58977] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.![PHF22 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: PHF22 Antibody - BSA Free [NBP2-58977]](https://resources.bio-techne.com/images/products/nbp2-58977_rabbit-polyclonal-phf22-antibody-2562025913916.jpg "Chromatin Immunoprecipitation-exo-Seq: PHF22 Antibody - BSA Free [NBP2-58977]")
Chromatin Immunoprecipitation-exo-Seq: PHF22 Antibody - BSA Free [NBP2-58977]
ChIP-Exo-Seq composite graph for Anti-INTS12 (NBP2-58977) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for PHF22 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PHF22
Alternate Names
Int12, integrator complex subunit 12, PHD finger protein 22INT12, PHF22, SBBI22
Gene Symbol
INTS12
Additional PHF22 Products
Product Documents for PHF22 Antibody - BSA Free
Product Specific Notices for PHF22 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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