PFDN2 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00279
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA, Immunoprecipitation
Cited:
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human PFDN2 (Uniprot#: Q9UHV9; Met1-Ser154)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for PFDN2 Antibody - Azide and BSA Free
Immunoprecipitation: PFDN2 Antibody [NBP3-00279] -
Immunoprecipitation: PFDN2 Antibody [NBP3-00279] - PFDN2 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 2 uL NBP3-00279 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00279, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 17 kDa Observed band size: 17 kDa
Western Blot: PFDN2 Antibody [NBP3-00279] -
Western Blot: PFDN2 Antibody [NBP3-00279] - NBP3-00279 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:17 kDa (We are unsure as to the identity of these extra bands.)
Western Blot: PFDN2 Antibody - Azide and BSA Free [NBP3-00279] -
PFDN2 promotes cell cycle progression in GC cells. (A) Bubble chart showing Gene Ontology (GO) enrichment analysis of the RNA-sequencing (RNA-seq) data from PFDN2-KD and HGC27-NC cells. (B) GSEA of the RNA-sequencing (RNA-seq) data from PFDN2-KD and HGC27-NC cells. (C) Flow cytometry was used to analyze the cell cycle in HGC27-NC and PFDN2-KD cell lines and in MKN45-NC and PFDN2-OE cell lines. (D) Cell proliferation abilities were determined by EdU staining in HGC27-NC and PFDN2-KD cell lines and in MKN45-NC and PFDN2-OE cell lines. (E) WB analysis of PFDN2, cyclin D1, cyclin E1 and CDK2 in HGC27-NC and PFDN2-KD cell lines and in MKN45-NC and PFDN2-OE cell lines. The protein levels were quantified with ImageJ. **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fonc.2023.1164070/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for PFDN2 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PFDN2
Alternate Names
PFD2, prefoldin 2, prefoldin subunit 2
Gene Symbol
PFDN2
Additional PFDN2 Products
Product Documents for PFDN2 Antibody - Azide and BSA Free
Product Specific Notices for PFDN2 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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