Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78790
![Western Blot: Peripheral Node Addressin Antibody (MECA-79)Low Endotoxin and Azide Free [NBP2-78790]](https://resources.bio-techne.com/images/products/Peripheral-Node-Addressin-Antibody-MECA-79-Low-Endotoxin-and-Azide-Free-Western-Blot-NBP2-78790-img0003.jpg "Western Blot: Peripheral Node Addressin Antibody (MECA-79)Low Endotoxin and Azide Free [NBP2-78790]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgM Kappa Clone # MECA-79
Format
Low Endotoxin and Azide Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Mouse lymph node stromal cells
Clonality
Monoclonal
Host
Rat
Isotype
IgM Kappa
Endotoxin Level
Endotoxin level is <0.02 EU/ug
Scientific Data Images
Western Blot: Peripheral Node Addressin Antibody (MECA-79)Low Endotoxin and Azide Free [NBP2-78790]
Western Blot: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790] - Total protein from human Tonsil, Lymph node, Spleen and mouse Spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-PNAd in blocking buffer and detected with an anti-rat HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image using the standard format of this product.![Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790]](https://resources.bio-techne.com/images/products/Peripheral-Node-Addressin-Antibody-MECA-79-Low-Endotoxin-and-Azide-Free-Immunohistochemistry-Paraffin-NBP2-78790-img0002.jpg "Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790]")
Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790]
Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790] - Analysis of FFPE mouse adipose tissue section (with lymph node areas) using Peripheral Node Addressin antibody (clone MECA-79) at 1:100. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This Peripheral Node Addressin antibody generated a strong and specific staining of MECA-79 antigen in the the cytoplasm and the membranes of high endothelial venules (HEVs) aka peripheral lymph node addressin (PNAd) in lymph node areas of tested adipose tissue section. Image using the standard format of this product.![Immunohistochemistry: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790]](https://resources.bio-techne.com/images/products/Peripheral-Node-Addressin-Antibody-MECA-79-Low-Endotoxin-and-Azide-Free-Immunohistochemistry-NBP2-78790-img0001.jpg "Immunohistochemistry: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790]")
Immunohistochemistry: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790]
Immunohistochemistry: Peripheral Node Addressin Antibody (MECA-79) - Low Endotoxin and Azide Free [NBP2-78790] - Analysis of FFPE human tonsil tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:100. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. Image using the standard format of this product.Applications
Application
Recommended Usage
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Frozen
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
1:10-1:500
In vivo assay
reported in scientific literature (PMID 30277476)
Western Blot
1:100-1:2000
Application Notes
Additional reported application of in vitro and in vivo blocking of cell adhesion.
Formulation, Preparation, and Storage
Purification
IgM purified
Formulation
PBS
Format
Low Endotoxin and Azide Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Background: Peripheral Node Addressin
Alternate Names
MECA-79, Peripheral Node Addressin, PNAd
Additional Peripheral Node Addressin Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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