Peripheral Node Addressin Antibody (MECA-79) - Non-Recombinant Monoclonal

Novus Biologicals, part of Bio-Techne | Catalog # NB100-77673

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgM Kappa Clone # MECA-79

Format

Non-Recombinant Monoclonal

Concentration

1.0 mg/ml

View all Peripheral Node Addressin Antibodies »

Product Specifications

Immunogen

Mouse lymph node stromal cells

Clonality

Monoclonal

Host

Rat

Isotype

IgM Kappa

Scientific Data Images

Immunohistochemistry: Peripheral Node Addressin Antibody (MECA-79) - Non-Recombinant Monoclonal [NB100-77673]

Immunohistochemistry: Peripheral Node Addressin Antibody (MECA-79) - Non-Recombinant Monoclonal [NB100-77673]

Peripheral-Node-Addressin-Antibody-MECA-79-Non-Recombinant-Monoclonal-Immunohistochemistry-NB100-77673-img0006.jpg
Western Blot: Peripheral Node Addressin Antibody (MECA-79)Non-Recombinant Monoclonal [NB100-77673]

Western Blot: Peripheral Node Addressin Antibody (MECA-79)Non-Recombinant Monoclonal [NB100-77673]

Western Blot: Peripheral Node Addressin Antibody (MECA-79) - Non-Recombinant Monoclonal [NB100-77673] - Total protein from human Tonsil, Lymph node, Spleen and mouse Spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-PNAd in blocking buffer and detected with an anti-rat HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image using the standard format of this product.
Western Blot: Peripheral Node Addressin Antibody (MECA-79)Non-Recombinant Monoclonal [NB100-77673]

Western Blot: Peripheral Node Addressin Antibody (MECA-79)Non-Recombinant Monoclonal [NB100-77673]

Western Blot: Peripheral Node Addressin Antibody (MECA-79) - Non-Recombinant Monoclonal [NB100-77673] - Total protein from Human tonsil (lane 1) and human lymph node (lane 2) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/mL anti-PNAd in 1% milk, and detected with an anti-rat IgM HRP conjugated secondary antibody using chemiluminescence.
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Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 31024011)

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

In vivo assay

reported in scientific literature (PMID 30277476)

Western Blot

1:100-1:2000
Application Notes
Additional reported application of in vitro and in vivo blocking of cell adhesion.

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Format

Non-Recombinant Monoclonal

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peripheral Node Addressin

MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.

Alternate Names

MECA-79, Peripheral Node Addressin, PNAd

Additional Peripheral Node Addressin Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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