PER2 Antibody (2K9Y0)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16557
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2K9Y0 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PER2 (O15055). MNGYAEFPPSPSNPTKEPVEPQPSQVPLQEDVDMSSGSSGHETNENCSTGRDSQGSDCDDSGKELGMLVEPPDARQSPDTFSLMMAKSEHNPSTSGCSSD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
137 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit PER2 Antibody (2K9Y0) (NBP3-16557) is a recombinant monoclonal antibody validated for use in WB, ICC/IF and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PER2 Antibody (2K9Y0)
![Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557]](https://resources.bio-techne.com/images/products/PER2-Antibody-2K9Y0-Western-Blot-NBP3-16557-img0003.jpg "Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557]")
Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557]
Western Blot: PER2 Antibody (2K9Y0) [NBP3-16557] - Western blot analysis of extracts of various cell lines, using PER2 Rabbit mAb (NBP3-16557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Chromatin Immunoprecipitation: PER2 Antibody (2K9Y0) [NBP3-16557] -
Chromatin Immunoprecipitation: PER2 Antibody (2K9Y0) [NBP3-16557] - Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using PER2 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.Applications for PER2 Antibody (2K9Y0)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PER2
Alternate Names
Circadian clock protein PERIOD 2, FASPS, hPER2, KIAA0347period 2, period (Drosophila) homolog 2, period circadian protein 2, period circadian protein homolog 2, period homolog 2 (Drosophila)
Gene Symbol
PER2
Additional PER2 Products
Product Documents for PER2 Antibody (2K9Y0)
Product Specific Notices for PER2 Antibody (2K9Y0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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