Pax2 Antibody (CL9469) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44202

Novus Biologicals, part of Bio-Techne
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NBP3-44202
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BSA Free
NBP2-88934
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL9469

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Pax2 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q02962, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Pax2 Antibody (CL9469) - Azide and BSA Free

Pax2 Antibody (CL9469) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Pax2 Antibody [NBP3-44202]

Immunocytochemistry/ Immunofluorescence: Pax2 Antibody [NBP3-44202]

Staining of EFO-21 cells using the Anti-Pax2 monoclonal antibody) .
Pax2 Antibody (CL9469) - Azide and BSA Free Immunohistochemistry-Paraffin: Pax2 Antibody [NBP3-44202]

Immunohistochemistry-Paraffin: Pax2 Antibody [NBP3-44202]

Analysis in human kidney and prostate tissues using NBP3-44202 antibody. Corresponding Pax2 RNA-seq data are presented for the same tissues.
Pax2 Antibody (CL9469) - Azide and BSA Free Immunohistochemistry-Paraffin: Pax2 Antibody [NBP3-44202]

Immunohistochemistry-Paraffin: Pax2 Antibody [NBP3-44202]

Staining of human kidney shows strong nuclear positivity in cells in distal tubules.
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Applications for Pax2 Antibody (CL9469) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pax2

PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.

Long Name

Paired Box Gene 2

Alternate Names

paired box 2, paired box gene 2, paired box homeotic gene 2, paired box protein Pax-2

Gene Symbol

PAX2

Additional Pax2 Products

Product Documents for Pax2 Antibody (CL9469) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pax2 Antibody (CL9469) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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