PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32784
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE57-42 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser474 of human PAK4. (Uniprot: O96013)
Modification
p Ser 474, p Ser 560, p Ser 602
Localization
Cytoplasm; Mitochondrion, Cytoplasm, Nucleus; Cytoplasm, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42) (NBP3-32784) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)
![PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)](https://resources.bio-techne.com/images/products/nbp3-32784_rabbit-pak4-5-6-p-ser602-p-ser474-p-ser560-mab-je57-42-288202412442919.jpg "Western Blot: PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42) [NBP3-32784]")
Western Blot: PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42) [NBP3-32784]
Western Blot: PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42) [NBP3-32784] - Western blot analysis of PAK4+5+6 on different lysates with Rabbit anti-PAK4+5+6 antibody (NBP3-32784) at 1/1,000 dilution.Lane 1: A549 cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: MCF7 cell lysate (20 ug/Lane)
Lane 4: HUVEC cell lysate (10 ug/Lane)
Lane 5: HUVEC treated with 50ng/mL VEGF for 2 minutes cell lysate (30 ug/Lane)
Predicted band size: 64/81/75 kDa
Observed band size: 64 kDa
Exposure time: 30 seconds; ECL;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32784) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PAK4+5+6
Alternate Names
EC 2.7.11, EC 2.7.11.1, KIAA1142, p21 protein (Cdc42/Rac)-activated kinase 4, p21(CDKN1A)-activated kinase 4, p21-activated kinase 4, PAK-4, protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs, serine/threonine-protein kinase PAK 4
Gene Symbol
PAK4
Additional PAK4+5+6 Products
Product Documents for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)
Product Specific Notices for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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