PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15234
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2D1
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Phospho-PAK4+5+6 (S474/S560/S602). UniProt (O96013/Q9P286/Q9NQU5).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) - BSA Free
Western Blot: PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) [NBP3-15234] - Positive WB detected in 293 whole cell lysate (treated with Calyculin A or not). All lanes PAK4+5+6 antibody at 2.15ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 64,75,80 KDa. Observed band size: 75,80 Kda.
Immunocytochemistry/Immunofluorescence: PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) [NBP3-15234] - Immunofluorescence staining of HepG2 cells (treated with 50mM Calyculin A for 30 minutes) with at PAK4+5+6 antibody 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Applications for PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: PAK4+5+6
Alternate Names
EC 2.7.11, EC 2.7.11.1, KIAA1142, p21 protein (Cdc42/Rac)-activated kinase 4, p21(CDKN1A)-activated kinase 4, p21-activated kinase 4, PAK-4, protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs, serine/threonine-protein kinase PAK 4
Gene Symbol
PAK4
Additional PAK4+5+6 Products
Product Documents for PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) - BSA Free
Product Specific Notices for PAK4+5+6 [p Ser474, p Ser560, p Ser602] Antibody (2D1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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