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P2Y1/P2RY1 Antibody (HL2702)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48720

Recombinant Monoclonal Antibody
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NBP3-48720

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2702

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human P2Y1/P2RY1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for P2Y1/P2RY1 Antibody (HL2702)

P2Y1/P2RY1 Antibody (HL2702)

Immunohistochemistry-Paraffin: P2Y1/P2RY1 Antibody (HL2702) [NBP3-48720] -

P2Y1/P2RY1 antibody [HL2702] detects P2Y1/P2RY1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreas.
P2Y1/P2RY1 stained by P2Y1/P2RY1 antibody [HL2702] (NBP3-48720) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
P2Y1/P2RY1 Antibody (HL2702)

Western Blot: P2Y1/P2RY1 Antibody (HL2702) [NBP3-48720] -

Boiled and unboiled LoVo whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y1/P2RY1 antibody [HL2702] (NBP3-48720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
P2Y1/P2RY1 Antibody (HL2702)

Western Blot: P2Y1/P2RY1 Antibody (HL2702) [NBP3-48720] -

Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y1/P2RY1 antibody [HL2702] (NBP3-48720) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for P2Y1/P2RY1 Antibody (HL2702)

Application
Recommended Usage

Western Blot

1:5000-1:50000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P2Y1/P2RY1

P2Y1 is a Purinergic Receptor that causes a change in platelet shape and potentially leads to platelet aggregation by binding ADP, which results in the mobilization of intracellular calcium ions via activation of phospholipase C. This receptor also mediates the release of the relaxing factor nitric oxide and may be involved in the modulation of neuronal signal transmission. P2Y1 has been reported to be expressed in tissues throughout the body, particularly brain. ESTs have been isolated from human embryo, lung, and placenta libraries.

Long Name

P2Y Purinergic Receptor 1

Alternate Names

P2RY1

Gene Symbol

P2RY1

Additional P2Y1/P2RY1 Products

Product Documents for P2Y1/P2RY1 Antibody (HL2702)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for P2Y1/P2RY1 Antibody (HL2702)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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