Nuf2 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99081

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nuf2 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Nuf2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Nuf2 Antibody - Azide and BSA Free

Western Blot: Nuf2 Antibody [NBP2-99081]

Western Blot: Nuf2 Antibody [NBP2-99081]

Western Blot: Nuf2 Antibody [NBP2-99081] - Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: Nuf2 Antibody [NBP2-99081]

Immunoprecipitation: Nuf2 Antibody [NBP2-99081]

Immunoprecipitation: Nuf2 Antibody [NBP2-99081] - Nuf2 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate. 1 ul anti-Nuf2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Nuf2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa. Observed band size: 51 kDa
Nuf2 Antibody - Azide and BSA Free

Western Blot: Nuf2 Antibody - Azide and BSA Free [NBP2-99081] -

Expression of NUF2 in ATC and clinical relevance.A The relevance of NUF2 expression and relapse-free survival (RFS) in patients with thyroid cancer (TC). B Expression of NUF2 in TC subgroups based on nodal metastasis status. C The expression of NUF2 in NT, PTC, PDTC, and ATC based on four microarray datasets from GEO database. D, E IHC staining of NUF2 in NT and ATC tissues. F–H The mRNA and protein levels of NUF2 in different thyroid cancer cell lines. I, J The expression of NUF2 in cytoplasm and nucleus. K The expression of NUF2 in different cell cycle phases. Data are presented as the mean +/- SD. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39242581), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Nuf2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nuf2

Nuf2, also known as Kinetochore protein Nuf2, is a 54 kDa 464 amino acid protein, and it is involved in chromosome segregation, specifically as it relates to spindle checkpoint activity with the kinetochore-associated NDC80 complex. This protein is necessary for kinetochore stability and organization of microtubule binding sites on the outer plate, forming interactions with NDC80/HEC-1-CDCA1, SPBC24-SPBC25, Bub1, EXOC1, as well as AURKB/Aurora-B and CENPE in the mitotic prometaphase, DNA replication, and Cell Cycle pathways. Current research is being performed on this proteins involvement in gastric, lung, and breast cancers, in addition to essential hypertension, schizophrenia, and duodenogastric reflux.

Alternate Names

cancer/testis antigen 106, CDCA1hsNuf2, cell division cycle associated 1, Cell division cycle-associated protein 1, CT106, hNuf2, kinetochore protein Nuf2, NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae), NUF2RhNuf2R

Gene Symbol

NUF2

Additional Nuf2 Products

Product Documents for Nuf2 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nuf2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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