Nucleobindin 1 Antibody (HL2532)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48694
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2532
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human Nucleobindin 1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Nucleobindin 1 Antibody (HL2532) (NBP3-48694) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Nucleobindin 1 Antibody (HL2532)
Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody (HL2532) [NBP3-48694] -
Nucleobindin 1 antibody [HL2532] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (NBP3-48694) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .
Western Blot: Nucleobindin 1 Antibody (HL2532) [NBP3-48694] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (NBP3-48694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Western Blot: Nucleobindin 1 Antibody (HL2532) [NBP3-48694] -
Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (NBP3-48694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for Nucleobindin 1 Antibody (HL2532)
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nucleobindin 1
Alternate Names
DKFZp686A15286, FLJ40471, NUCCALNUC, nucleobindin 1, nucleobindin-1
Gene Symbol
NUCB1
Additional Nucleobindin 1 Products
Product Documents for Nucleobindin 1 Antibody (HL2532)
Product Specific Notices for Nucleobindin 1 Antibody (HL2532)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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