Nuclear Antigen Antibody (235-1R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13912
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Primate
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 235-1R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Nuclei of human myeloid leukemia biopsy cells
Reactivity Notes
Does not react with Mouse, Rat and Chicken.
Localization
Nuclear
Specificity
This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Marker
Human Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
70,80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14102)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Nuclear Antigen Antibody (235-1R)
Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of PFA-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).
Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of PFA-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).
Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of MeOH-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).
Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of MeOH-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).Applications for Nuclear Antigen Antibody (235-1R)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Frozen
1-2 ug/ml
Immunoprecipitation
1000-2000 ug/500 ug protein lysate
Application Notes
Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT.
Immunohistology (Frozen): 1-2ug/ml for 30 minutes at RT
Immunoprecipitation: 1-2 g/500ug protein lysate)
Immunoprecipitation: 1-2 gram/500 ug protein lysate
Immunohistology (Frozen): 1-2ug/ml for 30 minutes at RT
Immunoprecipitation: 1-2 g/500ug protein lysate)
Immunoprecipitation: 1-2 gram/500 ug protein lysate
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Nuclear Antigen
Alternate Names
Nuclei, Nucleus
Additional Nuclear Antigen Products
Product Documents for Nuclear Antigen Antibody (235-1R)
Product Specific Notices for Nuclear Antigen Antibody (235-1R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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