Nuclear Antigen Antibody (235-1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34525

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Primate

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 235-1

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Nuclear Antigen Antibodies »

Product Specifications

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:35327471).Does not react with Mouse, Rat and Chicken.

Localization

Nuclear

Specificity

This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Marker

Human Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34342).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Nuclear Antigen Antibody (235-1) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525]

Immunocytochemistry/ Immunofluorescence: Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525]

Immunocytochemistry/Immunofluorescence: Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525] - Immunofluorescent staining of PFA-fixed MCF-7 cells with Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Green). Counterstained with Phalloidin (red).
Immunohistochemistry-Frozen: Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525]

Immunohistochemistry-Frozen: Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525]

Immunohistochemistry-Frozen: Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525] - Acetone-fixed frozen human Tonsil stained with Nuclear Antigen Antibody using HRP-DAB detection.
Flow Cytometry: Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525]

Flow Cytometry: Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525]

Flow Cytometry: Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525] - Flow Cytometric Analysis of PFA-fixed K562 cells with Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Applications for Nuclear Antigen Antibody (235-1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1.0ug/ml

Immunohistochemistry-Frozen

0.5-1.0 ug/ml

Immunoprecipitation

0.5-1ug/500ug protein lysate
Application Notes
Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT.
Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.
This antibody is not recommended for use with formalin fixed tissues.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Nuclear Antigen

Alternate Names

Nuclei, Nucleus

Additional Nuclear Antigen Products

Product Documents for Nuclear Antigen Antibody (235-1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nuclear Antigen Antibody (235-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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