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NSUN2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-47302

Novus Biologicals, part of Bio-Techne
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NBP3-47302

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NOL1/NOP2/Sun domain family, member 2. (Uniprot# Q08J23)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit NSUN2 Antibody - BSA Free (NBP3-47302) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NSUN2 Antibody - BSA Free

NSUN2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NSUN2 Antibody - BSA Free [NBP3-47302] -

Immunocytochemistry/ Immunofluorescence: NSUN2 Antibody - BSA Free [NBP3-47302] - Immunofluorescent analysis of Hela cells using (NSUN2 antibody) at dilution of 1:25
NSUN2 Antibody - BSA Free

Immunohistochemistry: NSUN2 Antibody - BSA Free [NBP3-47302] -

Immunohistochemistry: NSUN2 Antibody - BSA Free [NBP3-47302] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (NSUN2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(NSUN2
NSUN2 Antibody - BSA Free

Western Blot: NSUN2 Antibody - BSA Free [NBP3-47302] -

Western Blot: NSUN2 Antibody - BSA Free [NBP3-47302] - HepG2 cells cells were subjected to SDS PAGE followed by western blot with (NSUN2 antibody) at dilution of 1:5000

Applications for NSUN2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:20-1:200

Western Blot

1:500-1:2000
Application Notes
Theoretical Molecular Weight is 90-100 kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NSUN2

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].

Alternate Names

EC 2.1.1, EC 2.1.1.29, FLJ20303,5-methycytoisine methyltransferase, hTrm4, member 2, Myc-induced SUN-domain-containing protein, NOL1/NOP2/Sun domain family 2, NOL1/NOP2/Sun domain family member 2, NOP2/Sun domain family, member 2, SAKIMisu, Substrate of AIM1/Aurora kinase B, TRM4MISU, tRNA (cytosine-5-)-methyltransferase, tRNA (cytosine-5-)-methyltransferase NSUN2, tRNA methyltransferase 4 homolog

Gene Symbol

NSUN2

Additional NSUN2 Products

Product Documents for NSUN2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NSUN2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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