NRSF Antibody (CL0381) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42048

Novus Biologicals, part of Bio-Techne
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NBP2-42048
NBP2-42048-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0381

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all REST/NRSF Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q13127, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse NRSF Antibody (CL0381) - BSA Free (NBP2-42048) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NRSF Antibody (CL0381) - BSA Free

Immunocytochemistry/ Immunofluorescence: NRSF Antibody (CL0381) [NBP2-42048]

Immunocytochemistry/ Immunofluorescence: NRSF Antibody (CL0381) [NBP2-42048]

Immunocytochemistry/Immunofluorescence: NRSF Antibody (CL0381) [NBP2-42048] - Staining of U-2 OS cells using the Anti-REST monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048] - Staining of human cerebral cortex shows no nuclear positivity in neuronal cells as expected.
Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048]

Immunohistochemistry-Paraffin: NRSF Antibody (CL0381) [NBP2-42048] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
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Applications for NRSF Antibody (CL0381) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: REST/NRSF

REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.

Long Name

RE1-Silencing Transcription Factor

Alternate Names

NRSF, XBR

Gene Symbol

REST

Additional REST/NRSF Products

Product Documents for NRSF Antibody (CL0381) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NRSF Antibody (CL0381) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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