Nrf2 [p Ser40] Antibody (24GB4450)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50798

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NBP3-50798-100ul
NBP3-50798-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4450

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nrf2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-Nrf2 (S40) (Uniprot: Q16236)

Modification

p Ser40

Specificity

This antibody detects endogenous levels of NFE2L2 only when phosphorylated at S40

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nrf2 [p Ser40] Antibody (24GB4450)

Nrf2 [p Ser40] Antibody (24GB4450)

Immunocytochemistry/ Immunofluorescence: Nrf2 [p Ser40] Antibody (24GB4450)[NBP3-50798] -

Immunocytochemical staining of HepG2 cells with Nrf2 antibody (1:1,000). Nuclei were stained blue with DAPI; Nrf2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Nrf2 [p Ser40] Antibody (24GB4450)

Flow Cytometry: Nrf2 [p Ser40] Antibody (24GB4450)[NBP3-50798] -

Flow cytometric analysis of Nrf2expression in HepG2 cells using Nrf2 antibody (1:2,000). Green, isotype control; red, phospho-Nrf2 (Ser40).
Nrf2 [p Ser40] Antibody (24GB4450)

Western Blot: Nrf2 [p Ser40] Antibody (24GB4450)[NBP3-50798] -

Western blotting analysis using Nrf2 (Ser40) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for Nrf2 [p Ser40] Antibody (24GB4450)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nrf2

Nrf2 is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Long Name

NF-E2-related Factor 2

Alternate Names

NFE2L2

Gene Symbol

NFE2L2

Additional Nrf2 Products

Product Documents for Nrf2 [p Ser40] Antibody (24GB4450)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nrf2 [p Ser40] Antibody (24GB4450)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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