NOX1 Antibody - Azide Free

Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546]

Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546] - Mouse kidney. NOX1 stained by NOX1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Flow Cytometry: NOX1 Antibody [NBP1-31546]

Flow Cytometry: NOX1 Antibody [NBP1-31546] - Nox-1 is present at the surface of resting platelets. Human platelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG control at 1:250 dilution for 30 minutes at room temperature. FITC-tagged anti-rabbit (secondary antibody) was added at 1:100 dilution and incubated for an additional 30 minutes. Platelets were then diluted 1:25 v/v in Tyrodes buffer and read using a BD Accuri flow cytometer. Image from verified customer review.

Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: JC B: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:5000.

Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE, antibody diluted at 1:2000.

Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: NOX1 Antibody [NBP1-31546]

Immunocytochemistry/Immunofluorescence: NOX1 Antibody [NBP1-31546] - Nox1 antibody detects Nox1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nox1 protein stained by NBP1-31546 diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546]

Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546] - NOX1 antibody detects NOX1 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.NOX1 antibody diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: NOX1 Antibody [NBP1-31546] -

Western Blot: NOX1 Antibody [NBP1-31546] - Blueberry polyphenol extract (BPE) did not affect the expression of NADPH oxidases (NOX) in angiotensin (Ang) II-treated human aortic endothelial cells (HAECs). HAECs were treated with 200 µg/mL of BPE for 1 h then treated with 200 nM of Ang II for 12 h. Protein expression of NOX1 (A,B), NOX2 (C,D), NOX4 (E,F), & NOX5 (G,H) were determined by Western blot. Quantification was performed using Image Lab (Bio-Rad Laboratories, Inc.). Data are expressed as mean ± SD from three (NOX1 & NOX4) & five (NOX2 & NOX5) independent experiments. Values that do not share the same letter are significantly different from each other (p ≤ 0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35453301), licensed under a CC-BY license. Not internally tested by Novus Biologicals.