Nociceptin Antibody (CL15085) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44174

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL15085

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Nociceptin Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q13519, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nociceptin Antibody (CL15085) - Azide and BSA Free

Nociceptin Antibody (CL15085) - Azide and BSA Free Immunohistochemistry-Paraffin: Nociceptin Antibody [NBP3-44174]

Immunohistochemistry-Paraffin: Nociceptin Antibody [NBP3-44174]

Analysis in human cerebral cortex and placenta tissues using NBP3-44174 antibody. Corresponding Nociceptin RNA-seq data are presented for the same tissues.
Nociceptin Antibody (CL15085) - Azide and BSA Free Immunohistochemistry-Paraffin: Nociceptin Antibody [NBP3-44174]

Immunohistochemistry-Paraffin: Nociceptin Antibody [NBP3-44174]

Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Nociceptin Antibody (CL15085) - Azide and BSA Free Immunohistochemistry-Paraffin: Nociceptin Antibody [NBP3-44174]

Immunohistochemistry-Paraffin: Nociceptin Antibody [NBP3-44174]

Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in a subset of neurons.
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Applications for Nociceptin Antibody (CL15085) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nociceptin

Nociceptin (Orphanin FQ, OFQ), a heptadecapeptide peptide, has been designated as an endogenous ligand for the ORL1 receptor. The amino acid sequence of nociceptin hashomology with other opioid peptides, especially the prodynorphin fragment dynorphin A, suggesting a close evolutionary relationship between the precursors. A diffuse distribution throughout the brain is seen with binding studies and in situ hybridization suggesting an extensive role for nociceptin in a multitude of CNS functions. Immunocytochemical localizations in rat spinal cord have demonstrated nociceptin abundance in superficial dorsal horn, lateral spinal nucleus and the region dorsal to the central canal. Nociceptin immunoreactivity was not affected by dorsal rhizotomy, indicating that in spinal cord the peptide is produced by central rather than primary afferent neurons. The localization is supported by its function in processing of nociceptive signals.

Alternate Names

nociceptin, nocistatin, OFQ, P, PPNOC, prepronociceptin, propronociceptin

Gene Symbol

PNOC

Additional Nociceptin Products

Product Documents for Nociceptin Antibody (CL15085) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nociceptin Antibody (CL15085) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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