NLK Antibody (6G5V10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15728
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6G5V10 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human NLK (NP_057315.3). AVHLLCRMLVFDPSKRISAKDALAHPYLDEGRLRYHTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVRQVKEIIHQFILEQQKGNRVPLCINP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit NLK Antibody (6G5V10) (NBP3-15728) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for NLK Antibody (6G5V10)
![Western Blot: NLK Antibody (6G5V10) [NBP3-15728]](https://resources.bio-techne.com/images/products/NLK-Antibody-6G5V10-Western-Blot-NBP3-15728-img0002.jpg "Western Blot: NLK Antibody (6G5V10) [NBP3-15728]")
Western Blot: NLK Antibody (6G5V10) [NBP3-15728]
Western Blot: NLK Antibody (6G5V10) [NBP3-15728] - Analysis of extracts of various cell lines, using NLK antibody (NBP3-15728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.![Immunohistochemistry-Paraffin: NLK Antibody (6G5V10) [NBP3-15728]](https://resources.bio-techne.com/images/products/NLK-Antibody-6G5V10-Immunohistochemistry-Paraffin-NBP3-15728-img0001.jpg "Immunohistochemistry-Paraffin: NLK Antibody (6G5V10) [NBP3-15728]")
Immunohistochemistry-Paraffin: NLK Antibody (6G5V10) [NBP3-15728]
Immunohistochemistry-Paraffin: NLK Antibody (6G5V10) [NBP3-15728] - Human placenta using NLK Rabbit mAb (NBP3-15728) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for NLK Antibody (6G5V10)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NLK
Alternate Names
DKFZp761G1211, EC 2.7.11, EC 2.7.11.24, FLJ21033, LAK1, nemo like kinase, nemo-like kinaseserine/threonine-protein kinase NLK, Protein LAK1
Gene Symbol
NLK
Additional NLK Products
Product Documents for NLK Antibody (6G5V10)
Product Specific Notices for NLK Antibody (6G5V10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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