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NFATC1/NFAT2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57739

Novus Biologicals, part of Bio-Techne
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NBP2-57739-25ul
NBP2-57739

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GYGAALDGGPAGYFLSSGHTRPDGAPALESPRIEITSCLGLYHNNNQFFHDVEVEDVLPSSKRSPSTATLSLPSLEAYRD

Reactivity Notes

Rat 84%. Use in Mouse reported in secitific publication PMID: 32729236

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit NFATC1/NFAT2 Antibody - BSA Free (NBP2-57739) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-NFATC1/NFAT2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NFATC1/NFAT2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody [NBP2-57739]

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody [NBP2-57739]

Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody [NBP2-57739] - Staining of human cell line MCF7 shows localization to nucleoplasm & nuclear bodies.
NFATC1/NFAT2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: NFATC1/NFAT2 Antibody - BSA Free [NBP2-57739]

Chromatin Immunoprecipitation-exo-Seq: NFATC1/NFAT2 Antibody - BSA Free [NBP2-57739]

ChIP-Exo-Seq composite graph for Anti-NFATC1 (NBP2-57739) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for NFATC1/NFAT2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF, Fixation/Permeabilization: PFA/Triton X-100

Reviewed Applications

Read 1 review rated 3 using NBP2-57739 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFATC1

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that coordinate the immune response. NFAT2 is the cytosolic component of NFAT and is encoded by a separate gene. Expression of NFAT2 activates the interleukin (IL-2) promoter in non-T lymphocytes and is required for IL-2 gene expression.

Long Name

Nuclear Factor of Activated T Cells C1

Alternate Names

NFAT2

Gene Symbol

NFATC1

Additional NFATC1 Products

Product Documents for NFATC1/NFAT2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NFATC1/NFAT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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