Neuron-specific beta-III Tubulin NL637 Antibody (Clone TuJ-1)

Detection of Neuron-specific beta-III Tubulin NL637 by Western Blot

ACY-738 induced changes in Ki67 and TUJ1 are not observed in ARL13B-KO, cilia-depleted human glioma cells. (A–P) S7 parental cells (left two columns) or ARL13B KO (clone G12) (right two columns) were grown to ~75% confluency and treated with vehicle or 500-nM 738. Cells were fixed after 24 h and immunostained for Ki67 (A–D), TUJ1 (E–H), and ARL13B (I–L). Merged images are displayed M-P. Scale bar in A = 10 µm. (Q) Mean nuclear intensity of Ki67 signal for indicated cell lines and treatments. n = 60–80 cells/group. * p < 0.05, **** p < 0.0001, ANOVA. (R) Mean soma intensity of TUJ1 signal for indicated cell lines and treatments. (S,T) Western blot of S7 (S) and L0 (T) cell lysates 24 h after exposure to vehicle or 500-nM 738. Blots were probed for TUJ1 and beta-actin as loading controls. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33915983), licensed under a CC-BY license. Not internally tested by R&D Systems.