Neuron-specific beta-III Tubulin APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC1195A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # TuJ-1
Product Specifications
Immunogen
Rat brain-derived microtubules
Specificity
Detects mammalian and chicken neuron-specific beta-III tubulin but not other beta-tubulin isotypes in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Neuron-specific beta-III Tubulin APC-conjugated Antibody
Detection of beta-III Tubulin in A172 cells by Flow Cytometry
A172 cells were stained with Mouse Anti-Neuron-specific beta-III Tubulin APC-conjugated Monoclonal Antibody (Catalog # IC1195A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.
Detection of beta‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry.
Eight-day differentiated Rat cortical stem cells was stained with Mouse Anti-beta -III Tubulin APC-conjugated Monoclonal Antibody (Catalog # IC1195A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Neuron-specific beta-III Tubulin APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: A172 human glioblastoma cell line and Eight-day differentiated Rat cortical stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: A172 human glioblastoma cell line and Eight-day differentiated Rat cortical stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta-III Tubulin
Alternate Names
beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III
Entrez Gene IDs
10381 (Human)
Gene Symbol
TUBB3
Additional beta-III Tubulin Products
Product Documents for Neuron-specific beta-III Tubulin APC-conjugated Antibody
Product Specific Notices for Neuron-specific beta-III Tubulin APC-conjugated Antibody
For research use only
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