NEK1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36534
![Western Blot: NEK1 Antibody [NBP2-36534]](https://resources.bio-techne.com/images/products/NEK1-Antibody-Western-Blot-NBP2-36534-img0004.jpg "Western Blot: NEK1 Antibody [NBP2-36534]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by the antibody maps to a region between residue 1208 to 1258 of human Never in Mitosis A-Related Kinase 1 using the numbering given in entry NP_036356.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NEK1 Antibody - BSA Free
Western Blot: NEK1 Antibody [NBP2-36534]
Western Blot: NEK1 Antibody [NBP2-36534] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiNEK1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for NEK1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
Negative
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NEK1
Alternate Names
DKFZp686D06121, DKFZp686K12169, EC 2.7.11, EC 2.7.11.1, KIAA1901MGC138800, Never in mitosis A-related kinase 1, NIMA (never in mitosis gene a)-related kinase 1, NimA-related protein kinase 1, NY-REN-55, protein-serine/threonine kinase, Renal carcinoma antigen NY-REN-55, serine/threonine-protein kinase Nek1, SRPS2
Gene Symbol
NEK1
UniProt
Additional NEK1 Products
Product Documents for NEK1 Antibody - BSA Free
Product Specific Notices for NEK1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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