NCOA2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22162
![Western Blot: NCOA2 Antibody [NBP2-22162]](https://resources.bio-techne.com/images/products/NCOA2-Antibody-Western-Blot-NBP2-22162-img0007.jpg "Western Blot: NCOA2 Antibody [NBP2-22162]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 1400 the C-terminus (residue 1464) of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit NCOA2 Antibody - BSA Free (NBP2-22162) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for NCOA2 Antibody - BSA Free
Western Blot: NCOA2 Antibody [NBP2-22162]
Western Blot: NCOA2 Antibody [NBP2-22162] - Western Blot: NCOA2 Antibody - hole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162]](https://resources.bio-techne.com/images/products/NCOA2-Antibody-Immunohistochemistry-Paraffin-NBP2-22162-img0009.jpg "Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162]")
Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162]
Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162] - Section of human testicular seminoma. Antibody: Affinity purified rabbit antiNCOA2/ SRC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.![Immunoprecipitation: NCOA2 Antibody [NBP2-22162]](https://resources.bio-techne.com/images/products/NCOA2-Antibody-Immunoprecipitation-NBP2-22162-img0008.jpg "Immunoprecipitation: NCOA2 Antibody [NBP2-22162]")
Immunoprecipitation: NCOA2 Antibody [NBP2-22162]
Immunoprecipitation: NCOA2 Antibody [NBP2-22162] - Immunoprecipitation: NCOA2 Antibody - Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells. Antibody: Affinity purified rabbit NCOA2 used at 20 ug/10 mg lysate. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2.Applications for NCOA2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
1-4ug/mg of Lysate
Western Blot
1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NCOA2
Long Name
Nuclear Receptor Co-activator 2
Alternate Names
bHLHe75, HTIF2, KAT13C, TIF2
Entrez Gene IDs
64708 (Human)
Gene Symbol
NCOA2
UniProt
Additional NCOA2 Products
Product Documents for NCOA2 Antibody - BSA Free
Product Specific Notices for NCOA2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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