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Napsin A Antibody (CL12954) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44369

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL12954

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of O96009, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Napsin A Antibody (CL12954) - Azide and BSA Free (NBP3-18551) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Napsin A Antibody (CL12954) - Azide and BSA Free

Napsin A Antibody (CL12954) - Azide and BSA Free Immunohistochemistry-Paraffin: Napsin A Antibody [NBP3-44369]

Immunohistochemistry-Paraffin: Napsin A Antibody [NBP3-44369]

Analysis in human kidney and skeletal muscle tissues using NBP3-44369 antibody. Corresponding Napsin A RNA-seq data are presented for the same tissues.
Napsin A Antibody (CL12954) - Azide and BSA Free Immunohistochemistry-Paraffin: Napsin A Antibody [NBP3-44369]

Immunohistochemistry-Paraffin: Napsin A Antibody [NBP3-44369]

Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
Napsin A Antibody (CL12954) - Azide and BSA Free Immunohistochemistry-Paraffin: Napsin A Antibody [NBP3-44369]

Immunohistochemistry-Paraffin: Napsin A Antibody [NBP3-44369]

Staining of human cerebral cortex shows no positivity in neurons as expected.

Applications for Napsin A Antibody (CL12954) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:5000 - 1:10000
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Napsin A

Napsin is a pepsin-like aspartic proteinase, in the A1 clan of the AA clade of proteinases. Other AA family proteinases include Pepsin, cathepsin-D, cathepsin-E and rennin. Of these proteinases, napsin is most closely related to cathepsin-D, sharing 46% identity at the amino acid level. There are two closely related Napsins, napsin A and napsin B, with 85% overall identity at the amino acid level. There are three isoforms. Napsin-A is also termed napsin-1, or TA02, a Tumor Adenocarcinoma marker. Napsin A may be involved in processing of pneumocyte surfactant precursors.

Alternate Names

ASP4, KAP, NAPA, NAPSA, Napsin-1, SNAPA, TA01/TA02

Gene Symbol

NAPSA

Additional Napsin A Products

Product Documents for Napsin A Antibody (CL12954) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Napsin A Antibody (CL12954) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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