N6-methyladenosine (m6A) Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18690
Key Product Details
Species Reactivity
Human, Mouse
Applications
Dot Blot, RNA immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Format
BSA Free
Concentration
1.2 mg/ml
Product Specifications
Immunogen
Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for N6-methyladenosine (m6A) Antibody - BSA Free
Dot Blot: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 4. Dot blot analysis using the antibody directed against N6-methyladenosine (m6A) To demonstrate the specificity of the antibody against N6-methyladenosine (m6A) , a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligos were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.
RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 1. RNA immunoprecipitation using the antibody directed against N6-methyladenosine (m6A) RNA Immunoprecipitation was performed on 40 ug HeLa total RNA spiked with 0.5 ug of an in vitro prepared transcript containing N6-methyladenosine (m6A) nucleotides, using 2 ug of the N6-methyladenosine (m6A) antibody . An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody [NBP3-18690] - Figure 3. RIP-seq results obtained with the antibody directed against N6-methyladenosine (m6A) RIP was performed with 2 ug of the antibody against N6-methyladenosine (m6A) . The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).
Applications for N6-methyladenosine (m6A) Antibody - BSA Free
Application
Recommended Usage
Dot Blot
1:400
RNA immunoprecipitation
1-2 ug/IP
Application Notes
Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 ug per IP.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide and 0.05% ProClin 300
Concentration
1.2 mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: N6-methyladenosine (m6A)
Alternate Names
(-)-6-(Methylamino)purine riboside, N(6)-Methyladenosine, N-Methyladenosine
Additional N6-methyladenosine (m6A) Products
Product Documents for N6-methyladenosine (m6A) Antibody - BSA Free
Product Specific Notices for N6-methyladenosine (m6A) Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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