Myosin light chain kinase Antibody (28C5) - BSA Free

Western Blot: Myosin light chain kinase Antibody (28C5) [NBP3-26122] -

Western Blot: Myosin light chain kinase Antibody (28C5) [NBP3-26122] - Positive Western Blot detected in: HepG2 whole cell lysate, HEK293 whole cell lysate.
All lanes: Myosin light chain kinase Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 211, 204, 206, 198, 203, 111, 17, 81 kDa
Observed band size: 130-250 kDa

Flow Cytometry: Myosin light chain kinase Antibody (28C5) [NBP3-26122] -

Flow Cytometry: Myosin light chain kinase Antibody (28C5) [NBP3-26122] - Overlay Peak curve showing A549 cells stained with Myosin light chain kinase Antibody (28C5) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.